Fluorescent silicon nanoparticle–based quantitative hemin assay

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Journal:Analytical and Bioanalytical Chemistry

Key Words:Free hemin Chiral silicon nanoparticles  Serum  Circular dichroism spectrum Dynamic quenching Inner flter efect

Abstract:Hemin with functions such as oxygen carrying, oxygen storing, promoting redox, and performing electron transfer is important for the health of organisms. In this paper, green synthetic silicon nanoparticles (Si-NPs) were synthesized and used for free hemin detection in serum (a low limit of detection (LOD), 29.5 nM). The quenching mechanism was investigated by UV–vis absorption spectra, time-resolved luminescence decay curve, and circular dichroism (CD) spectra. It was confrmed that multiple redox centers of hemin led to intensifed efective collision and increased the electron transfer rate, therefore enhancing the dynamic quenching, and it was undeniable that the inner flter efect (IFE) also played a role in the quenching.

Indexed by:SCI

Translation or Not:no

Date of Publication:2022-12-01

Included Journals:SCI